THE EFFECT OF THE CSN3 GENOTYPE ON THE CONTENT OF FATTY ACIDS IN MILK

In dairy cows, acids of different compositions represent the typical composition of fatty acids, about 70% of them are saturated fatty acids (SFA), 25% monounsaturated fatty acids (MUFA) and 5% polyunsaturated fatty acids (PUFA), which significantly differs from the ideal profile of fatty acids for human health. The analysis of methyl esters of fatty acids was carried out using a Shimadzu GC 2010 Plus gas chromatograph with a flame ionization detector (PID), also with a capillary column "CPSil 88 for FAME" (Agilent Technologies) with a length of 100 m, an inner diameter of 0.25 mm, and a film thickness of 0.20 microns of non-mobile phase.To determine the polymorphism of kappa-casein genes and evaluate economically useful traits in animals with different genotypes of kappa-casein, only 60 cows were selected, including 20 Holstein cows, 20 Alatau cows and 20 black-and-white cows. The polymorphism of kappa-casein genes was evaluated by PCR analysis. For further research, 3 subgroups of cows were formed in each group according to the principle of analogues, the results of genotyping and kappa-casein gene. The first group included cows with the AA kappa-casein genotype, the second – the AB genotype, and the third – the BB genotype.